Development of Poly Lactic/Glycolic Acid (PLGA) Microspheres for Controlled Release of Rho-Associated Kinase Inhibitor

Figure 4

Histological evaluation of corneal endothelium in rabbit eyes injected with poly lactic/glycolic acid (PLGA) microspheres. One or 10 mg PLGA microspheres was injected into the anterior chamber, and 1 mg PLGA microspheres was injected into the subconjunctiva. After 14 days, corneal endothelium was evaluated by immunofluorescence staining and phalloidin staining. Na⁺/K⁺-ATPase (a marker of pump function), ZO-1 (a marker of tight junction), and connexin 43 (a marker of gap junctions) were used to evaluate the functional property of corneal endothelium. Phalloidin staining was used for morphological analysis. Nuclei were stained with DAPI. Scale bar: 50 μm.