Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease

Figure 1

Pathological findings and confocal microscopic images of human cGVHD-affected lacrimal gland and conjunctival epithelia. Hematoxylin & eosin (H&E) staining. GVHD, case 3 (a); Sjögren’s syndrome (SS), case 2 (b). Transmission electron microscopic (TEM) findings. GVHD, case 2 (c); SS, case 1 (d). (c, d) Red arrows, secretory vesicle immunostaining for VAMP8 and E-cadherin expression in conjunctival epithelia (e, f) and lacrimal gland (g, h) from cGVHD (case 3) (e, g) and SS (case 2) (f, h) patient. VAMP8 is shown in red, E-cadherin in green, and 4,6-diamidino-2-phenylindole (DAPI) in blue. Scale bar = 50 μm (a), 100 μm (b), 4 μm (c, d), 25 μm (e, f), and 20 μm (g, h).