Characterization of Ex Vivo Expanded Oral Mucosal Epithelium Cells on Acellular Porcine Corneal Stroma for Ocular Surface Reconstruction

Figure 5

Immunohistochemistry of ex vivo expanded oral epithelium cultured from an explanted culture on either APCS or HAM. Multilayered confluent sheet of COMECs appeared after being cultured three to four weeks, which are well attached on APCS and HAM. It shows the expression of p63 in the nuclei of the basal epithelial sheet and ABCG2 and CK3 expression in the cytoplasm in the full-thickness in cultivated oral mucosal epithelial cell sheets both on APCS or HAM. But ABCG2 and p63 are expressed in the basal layer and CK3 is expressed in full-thickness in rat oral mucosal epithelial cells.