Assessment of bleb wall volume and intensity using 3D AS-OCT. (a) A selected imaging slice from all images of the horizontal raster. (b) A selected imaging slice that was automatically color-coded into four categories based on the optical density: (1) high intensity (red; optical density, 150–255); (2) medium intensity (yellow; optical density, 100–149); (3) low intensity (green; optical density, 50–99); and (4) fluid cavity (blue; optical density, 0–49), using built-in software. (c) A selected imaging slice with color code only in the bleb wall, which was defined to be subjected to analysis by manually drawing lines perpendicular to the scleral flap at the edges of the fluid cavity. (d) Schematic image of the three-dimensional reconstruction of the selected imaging slices after color-coding and bleb wall definition. (e) En-face, vertical, horizontal, and C-scan images after reconstruction with color code in the bleb wall. The red and blue lines indicate the horizontal and vertical axes, respectively. The yellow line represents the z-axis of the C-scan images. The volumes of each color-coded category were automatically calculated by reconstructive accumulation of the two-dimensional images, using the built-in software.