Research Article
Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering
Figure 1
Remained cellular components after decellularization. Remained nuclear and cell component were visualized with DAPI and vimentin after decellularized with hypotonic solution containing 0.5% Triton X-100 (a and b), 0.5% SDS (c and d), and 0.5% TE (e and f). The lenticules were cross sectioned for precise observation, respectively (b, d, and f). DAPI and vimentin stain of TE decellularized lenticules showed no positive signal. Original magnification is ×200 (a, c, and e) and ×400 (b, d, and f). Scale bar indicates 50 μm.
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