Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering

Figure 5

In vitro biocompatibility of DCL treated under various conditions. Human keratocyte attachment, spreading, and proliferating activity on the DCLs were evaluated. The cells were seeded on DCL treated with 0.5% TE under various tonic conditions and visualized with DAPI (blue) and phalloidin (green). The count and spreading area of attached cells in TE-treated DCL was higher than on SDS-treated DCL (b and c). Additionally, the proliferating activity of keratocytes on DCLs showed the highest rate in hypotonic TE (0.25 and 0.5%). The values shown are the means ± SEMs (). Statistical significance: , , and versus hypotonic 0.5% TE. n.s is not significant.

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