Research Article
Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering
Table 3
Reverse transcript PCR primers.
| | Gene | Sequence | Annealing °C | Cycles |
| | GAPDH | 5-GAGTCAACGGATTTGGTCGT-3 | 54°C | 20 | | 5-TTGATTTTGGAGGGATCTCG-3 | | ABCG2 | 5-GTTTATCCGTGGTGTGTCTGG-3 | 58°C | 20 | | 5-CTGAGCTATAGAGGCCTGGG-3 | | | Vimentin | 5-GAGAACTTTGCCGTTGAAGC-3 | 56°C | 20 | | 5-TCCAGCAGCTTCCTGTAGGT-3 | | | CK1 | 5-AGGAGGTGGACGTGGTAGTG-3 | 54°C | 30 | | 5-AGGAGGCAAATTGGTTGTTG-3 | | | CK3 | 5-GGCAGAGATCGAGGGTGTC-3 | 59°C | 30 | | 5-GTCATCCTTCGCCTGCTGTAG-3 | | | CK4 | 5-CTACAACCTCAGGGGGAACA-3 | 55°C | 30 | | 5-GCTCAAGGTTTTTGCTGGAG-3 | | | CK5 | 5-CTTGTGGAGTGGGTGGCTAT-3 | 56°C | 30 | | 5-CCACTTGGTGTCCAGAACCT-3 | | | CK12 | 5-ACATGAAGAAGAACCACGAGGATG-3 | 56°C | 30 | | 5-TCTGCTCAGCGATGGTTTCA-3 | | | CK13 | 5-GATCCAGGGACTCATCAGCA-3 | 56°C | 30 | | 5-AAGGCCTACGGACATCAGAA-3 | | | CK14 | 5-TTCTGAACGAGATGCGTGAC-3 | 55°C | 30 | | 5-GCAGCTCAATCTCCAGGTTC-3 | | | CK15 | 5-GGAGGTGGAAGCCGAAGTAT-3 | 58°C | 30 | | 5-GAGAGGAGACCACCATCGCC-3 | |
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