Inhibition of Proteasome Activity Upregulates IL-6 Expression in RPE Cells through the Activation of P38 MAPKs

Figure 7

Inhibition of p38 MAPK blocks proteasome inhibition-induced upregulation of IL-6. (a) ARPE-19 cells were cultured in the presence or absence of MG132 (10 μM), SB203580 (10 μM), or MG132 plus SB203580 for 2, 4, 6, 8, 10, and 12 h. Levels of IL-6 in the media were determined by ELISA. The results are the mean ± SD. and as compared with the control; as compared with the control or SB203580 alone. (b) Levels of IL-6 in the medium were detected by ELISA at 0 h–6 h and 6 h–12 h in the presence or absence of MG132 (10 μM), SB203580 (10 μM), or MG132 plus SB203580. The results are the mean ± SD. and as compared with the control; ^# as compared with the control or SB203580 alone.

(a)

(b)