Efficacy of Osthole in Management of Hypoperfused Retina
Immunohistochemical staining of NF-κB, Akt, and Bcl-2/Bax. Semiquantitative analysis of NF-κB (a), Akt (b), and Bcl-2/Bax (c) levels by IOD/area. NF-κB expression was significantly lower in the osthole-IG group than in the other groups between days 5 and 15. NF-κB expression was significantly lower in the osthole-PI group than in the model group on day 5 but not on day 15 (a); Akt expressions were significantly lower in the osthole-IG group than in the other groups (b). Bcl-2/Bax expression was significantly higher in the osthole-IG group than in the other groups (c). Parallel changes in Akt staining were observed (b). For the Bcl-2/Bax IOD ratio, the osthole-PI group result was higher than any other group on days 5 and 10 but significantly lower on day 15. In the osthole-IG group, this ratio continued to increase over time. In the other groups, fluctuating results were observed, but the Bcl-2/Bax IOD ratio was lower than that in the osthole-IG group in day 15 (c).