Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases

Table 1

Primers for quantitation of VEGF, bFGF, and PEDF.

Target	Primer’s sequence (5-3) (forward and reverse) and length (bp)	Amplicon size (bp)
VEGF	(F) AGATCGAGTACATCTTCAAGCCATC (25)	66
	(R) CGTCATTGCAGCAGCCC (17)
bFGF	(F) CCGACGGCCGAGTTGAC (17)	112
	(R) TAACGGTTAGCACACACTCCTTTG (24)
PEDF	(F) CGACCAACGTGCTCCTGTCT (20)	131
	(R) GATGTCTGGGCTGCTGATCA (20)
GAPDH	(F) CATCCATGACAACTTTGGTATCGT (24)	74
	(R) CAGTCTTCTGGGTGGCAGTGA (21)

VEGF: vascular endothelial growth factor; bFGF: basic fibroblast growth factor; PEDF: pigment epithelium-derived factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.