Increasing Donor Endothelial Cell Pool by Culturing Cells from Discarded Pieces of Human Donor Corneas for Regenerative Treatments

Figure 1

Peel and digest method. (a) After fixing the tissue on a vacuum block, an 8.25 mm Moria trephine (Moria, Antony, France) was used to gently tap the endothelial side on its surface to create a superficial cut, which was identified using trypan blue staining. (b) The peripheral 2.75 mm zone was gently detached using 120 mm acute forceps (e.janach, Como, Italy), (c) followed by peeling the central 8.25 mm zone.

(a)

(b)

(c)