Journal of Ophthalmology / 2019 / Article / Fig 2

Research Article

Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy

Figure 2

Effects of MG and glucose treatment on retinal angiogenesis in 4 dpf zebrafish. Zebrafish embryos were incubated with solution containing MG with or without glucose from 10 hpf to 4 dpf. (a) Fluorescence microscope observations of the retinal vessels of drug-treated embryos are shown at 4 dpf. The 1000 µM MG treatment with or without 30 mM glucose group induced retinal vessel branching and vascular area compared with the untreated control in zebrafish embryos. As the positive control, zebrafish embryos treated with VEGF inducer GS4012 (1 µM and 3 µM) showed severe branching and disorganization in the retinal vasculature. Quantification of the percentage of vascular area (b) and the number of branch points (c) in midperiphral retina by MG, glucose, and GS4012 treatment in zebrafish embryos at 4 dpf. n = 30 eyes of 15 zebrafish embryos per group; mean ± SEM. and vs control group. Data were analyzed using the one-way ANOVA and then Student’s t-test. Glu, glucose. Ctl, control.

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