Journal of Ophthalmology / 2019 / Article / Fig 3

Research Article

Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy

Figure 3

Effect of VEGF receptor inhibitor (PTK787) in MG-induced zebrafish. (a) Fluorescence microscope observations shown added 0.5 µM PTK787 in 1 dpf in the 1000 µM MG and 30 mM glucose treatment group reverted blood vessel hyperbranching in zebrafish embryos at 4 dpf. The proangiogenic effect induced by MG treatment was inhibited and exhibited no significant difference compared with the no-treatment control. Quantification of the percentage of vascular area (b) and number of branch points (c) in midperiphral retina by PTK787, MG, and glucose treatment in zebrafish embryos at 4 dpf. n = 30 eyes of 15 zebrafish embryos per group; mean ± SEM. , vs control group. nonsignificant (NS). Data were analyzed using the one-way ANOVA and then Student’s t-test. Glu, glucose. CTL, control.

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