Figure 1: (a) Schematic diagram of the high-magnification digital microscopy-based approach for quantitative characterization of IOL vacuoles: O, high-magnification (75x to 5000x) microscope objective; CCD, digital camera; IOL, test IOL sample placed at in situ simulation conditions; TS, x-y-z translational stage. (b) 100 μm calibration scale with a minimum spatial resolution of 2 μm measured with the digital microscope at 150x (upper picture) and 2000x (lower picture) magnification, along with the measured FOV.