Research Article

ROCK Inhibitor-Induced Promotion of Retinal Pigment Epithelial Cell Motility during Wound Healing

Figure 4

The effect of 10 μM Y27632 on RPE cell proliferation. (a) Time course of the number of viable RPE cells treated with and without Y24632 at 2, 3, and 7 days after seeding. (b) Phase-contrast images of RPE cells treated with (right) and without (left) Y24632 at 3 days after seeding. (c) Flow-cytometry chart of the cell cycle distribution in G1, S, and, G2/M phase in untreated RPE cells. (d) Flow-cytometry chart of the cell cycle distribution in G1, S, and, G2/M phase in synchronized untreated (upper) and Y24632-treated (lower) RPE cells before and at 2, 4, 6, 8, 12, 16, 24, and 32 hours after block release.