The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury

Figure 4

Assessment of retinal structure and fibrosis by Masson’s staining and immunohistochemistry staining. ((a)–(c)) Masson’s staining of rabbit eyes 28 days after OGI revealed bright blue staining at the wound site, indicating formation of fibrotic tissue. The bottom panel showed higher magnification of the boxed areas of the original image above. (d) Quantification of Masson’s staining. (e–g) Representative images of α-SMA staining of retinal cryosections. Eyes with OGI showed strong fluorescent signals at the wound site, mitigated by intravitreal anti-VEGF treatments. (h) Quantification of α-SMA fluorescent intensities shown in (e–g). Data are mean ± SD, n = 3 animals per group. versus OGI by one-way ANOVA with Tukey’s multiple comparisons test. Scale bar, 100 μm ((a–c) and (e–g)).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)