Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia

Figure 3

Analysis of HIF-1α and β-actin protein expression levels by western blot evaluated in MIO-M1 cells at different time points of treatment. (a) Representative immunoreactive bands for HIF-1α (top; 120 kDa) and β-actin (bottom; 43 kDa). For each sample, 200 μg of protein was loaded on SDS-8% polyacrylamide gel. (b) Relative expression of HIF-1α protein, normalized to the respective β-actin, is represented as mean ± SD of three repeated experiments.  < 0.001 (24 h CoCl₂ vs. 24 h CTR);  < 0.0001 (48 h CoCl₂ vs. 24 h CTR); and  < 0.0001 (48 h CoCl₂ vs. 48 h CTR) by one-way ANOVA and Tukey’s post hoc test.

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