Circ-ADAM9 Promotes High Glucose-Induced Retinal Pigment Epithelial Cell Injury in DR via Regulating miR-338-3p/CARM1 Axis

Figure 4

Effects of si-circ-ADAM9 and anti-miR-338-3p on HG-induced ARPE-19 cell injury. (a) The transfection efficiency of anti-miR-338-3p was assessed by detecting miR-338-3p expression. (b–j) ARPE-19 cells were transfected with si-NC, si-circ-ADAM9, si-circ-ADAM9 + anti-miR-NC, or si-circ-ADAM9 + anti-miR-338-3p and then treated with HG. The cells treated with NG were used as control. (b) The expression of miR-338-3p was determined using qRT-PCR. (c) The concentrations of IL-6 and TNF-α were determined by ELISA. MTT assay (d), EdU assay (e), and flow cytometry (f, g) were measured to assess cell proliferation and apoptosis. (h) The WB analysis was performed to detect the protein expression of Bax and cleaved-caspase-3. (i-j) MDA level and SOD activity were analyzed to evaluate cell oxidative stress. , , , and .

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