Retaining Residual Ovarian Tissue following Ovarian Failure Has Limited Influence on Bone Loss in Aged Mice

Figure 1

Temporal changes in lumbar BMD in aged mice. Aged C57BL/6Hsd female mice (9–12 months old) were dosed daily for 15 days with sesame oil (vehicle; CON) or VCD (160 mg/kg). An additional group of age-matched animals was not dosed but rather underwent bilateral ovariectomy (OVX) on d35 after the onset of dosing (matching ovarian failure in VCD-treated mice). BMD was determined using DXA as described in Section 2. Data are presented as percentage from baseline BMD $\pm$ SEM ($n=5\text{–}8$). ($\#$) and ($\$$) indicate $P<.05$ from baseline BMD for VCD and OVX groups, respectively, and (&) indicates $P<.05$ versus OVX. The dashed arrow indicates onset of ovarian failure in VCD-treated mice, and the dashed box indicates BMD values measured after ovarian failure had occurred.