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Journal of Osteoporosis
Volume 2012 (2012), Article ID 162806, 8 pages
Research Article

Detecting Early Biomechanical Effects of Zoledronic Acid on Femurs of Osteoporotic Female Rats

1Department of Orthopaedics and Trauma Surgery, Marilia State Medical School, FAMEMA, Marilia, SP 17519-030, Brazil
2Department of Surgery and Orthopaedics, Botucatu Medical School, UNESP, Botucatu, SP 18618-000, Brazil
3Department of Medical Education, Marilia State Medical School, FAMEMA, Marilia, SP 17519-030, Brazil

Received 3 June 2012; Revised 30 September 2012; Accepted 19 October 2012

Academic Editor: Jean Jacques Body

Copyright © 2012 Evandro Pereira Palacio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. To investigate the biomechanical effects of zoledronic acid (ZA) on femurs of female osteoporotic rats after follow-up periods of 9 and 12 months. Methods. Eighty female Wistar rats were prospectively assessed. At 60 days of age, the animals were randomly divided into two groups: bilateral oophorectomy (O) ( ) and sham surgery (S) ( ). At 90 days of age, groups O and S were randomly subdivided into four groups, according to whether 0.1 mg/kg of ZA or distilled water (DW) was intraperitoneally administered: OZA ( ), ODW ( ), SZA ( ), and SDW ( ). The animals were sacrificed at 9 and 12 months after the administration of the substances, and then their right femurs were removed and analyzed biomechanically. Axial compression tests that focused on determining the maximum load (N), yield point (N), and stiffness coefficient (N/mm) of the proximal femur were performed in the biomechanical study. Results. ZA significantly increased the maximum load and yield point, reducing the stiffness coefficient concerning the oophorectomy status and follow-up period. Conclusion. Zoledronic acid, at a dose of 0.1 mg/kg, significantly increased the maximum loads and yield points and reduced the stiffness coefficients in the femurs of female rats with osteoporosis caused by bilateral oophorectomy.