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Journal of Osteoporosis
Volume 2013, Article ID 760586, 10 pages
Research Article

Black Tea May Be a Prospective Adjunct for Calcium Supplementation to Prevent Early Menopausal Bone Loss in a Rat Model of Osteoporosis

1Pre-Clinical Physiology Laboratory, Tripura Institute of Paramedical Sciences, Hapania, Amtali, Tripura 799 130, India
2Formerly Department of Physiology, Presidency College, Kolkata, West Bengal, India
3Department of Physiology, Hooghly Mohsin College, Chinsurah, Hooghly, West Bengal, India
4Department of Physiology, Kalyani Mahavidyalaya, Kalyani, Nadia, West Bengal, India
5Department of Physiology, Serampore College, 9/1 William Carey Road, Serampore, Hooghly, West Bengal, India

Received 30 November 2012; Revised 10 June 2013; Accepted 20 June 2013

Academic Editor: Harri Sievänen

Copyright © 2013 Asankur Sekhar Das et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was undertaken to find out the ability of black tea extract (BTE) as a suitable alternative of adjunct for calcium supplementation in treating an ovariectomized rat model of early osteoporosis. Female Wistar rats weighing 140–150 g were divided into four groups consisting of six animals in each group: (A) sham-operated control; (B) bilaterally ovariectomized; (C) bilaterally ovariectomized + BTE; (D) bilaterally ovariectomized + 17β-estradiol. Results suggest that BTE could promote intestinal absorption of calcium significantly ( for duodenum and ileum; and for jejunum). This was found associated with enhanced activities of two relevant intestinal mucosal enzymes alkaline phosphatase ( for duodenum, jejunum, and ileum) and Ca2+ activated ATPase ( for duodenum, jejunum, and ileum). Such BTE-mediated promotion of calcium absorption was coupled with increase in serum estrogen titer ( ) and recovery of all urinary, bone, and serum osteoporotic marker parameters, including bone histological features. Serum parathyroid hormone level, however, was not altered in these animals ( ). A comparative study with 17β-estradiol, a well-known adjunct for calcium supplementation, indicated that efficacy of BTE in maintaining skeletal health is close to that of 17β-estradiol. This study suggests that simultaneous use of BTE is promising as a prospective candidate for adjunctive therapies for calcium supplementation in the early stage of menopausal bone changes.