Research Article

Prevention of Osteoporosis in the Ovariectomized Rat by Oral Administration of a Nutraceutical Combination That Stimulates Nitric Oxide Production

Figure 8

Effect of the incubation of COMB-4 on calcium deposition in differentiated osteoblastic MC3T3-E1 cell line. Panel (a): representative images of MC3T3-E1 cell line differentiated for ten days into osteoblasts and incubated with or without COMB-4 for one week. Calcium deposition after COMB-4 incubation done by Alizarin red S staining. Panel (b): quantitative measurements of Alizarin red S staining of the differentiated MC3T3-E1. Results are expressed as percent of positive Alizarin red area/total area and represent the mean ± SEM of three experiments. p < 0.05 respect to the control group.