Prevention of Osteoporosis in the Ovariectomized Rat by Oral Administration of a Nutraceutical Combination That Stimulates Nitric Oxide Production

Figure 9

Effect of the incubation of COMB-4 on cGMP expression in differentiated MC3T3-E1 cell line. After MC3T3-E1 cells were differentiated with ascorbic acid and β-glycerophosphate for seven days, osteoblast cells were incubated for 24 hours with or without COMB-4. cGMP expression was determined by ELISA. Results are expressed as pmol/mg of protein and represent the mean ± SEM of four experiments done in duplicate. p < 0.01 with respect to control.