Differential Effects of Chronic Pulsatile versus Chronic Constant Maternal Hyperglycemia on Fetal Pancreatic β-Cells

Figure 2

Fetal glucose-stimulated insulin secretion. A square-wave fetal hyperglycemic clamp was used to test insulin secretion. The average basal (minute −25, −15, −5) and hyperglycemic clamp (minute 60, 90, 120) glucose (a) and insulin concentrations (b) in control (𝑛=7, black bars), CHG (𝑛=6, white bars), and PHG (𝑛=5, gray bars) fetuses are shown. *, ** indicate a significant difference between basal and hyperglycemic clamp concentrations within a treatment group, 𝑃=0.029, 𝑃<0.0001, respectively. ● indicates a significant increase in the incremental change of glucose concentrations in CHG compared to both PHG and controls, 𝑃<0.0001. # indicates a significant decrease in the incremental change of insulin concentrations in CHG compared to both PHG and controls, 𝑃=0.0002.

(a)

(b)