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Journal of Pregnancy
Volume 2016, Article ID 4293431, 11 pages
http://dx.doi.org/10.1155/2016/4293431
Research Article

A Low-Protein Diet Enhances Angiotensin II Production in the Lung of Pregnant Rats but Not Nonpregnant Rats

Department of Obstetrics & Gynecology, Baylor College of Medicine, Houston, TX 77030, USA

Received 16 December 2015; Revised 15 March 2016; Accepted 28 March 2016

Academic Editor: Tamas Zakar

Copyright © 2016 Haijun Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The abundance of both ACE (Supplementary Figure 1(a)) and ACE2 (Supplementary Figure 1(b)) proteins in the lung was unchanged in nonpregnant rats fed LP diet compared to those fed CT diet at Days 8, 12, and 17 after start of diet treatment. Similarly, the abundance of both ACE (Supplementary Figure 2(a)) and ACE2 (Supplementary Figure 2(b)) proteins in the kidney was unchanged in nonpregnant rats fed LP diet compared to those fed CT diet at all 3 days investigated. The abundance of plasma ACE proteins was unchanged in LP rats compared to CT rats at Days 8 and 12 after start of diet treatment but reduced 2.2-fold (P < 0.01) at Day 17 (Supplementary Figure 3), while ACE2 was undetectable by western blotting.

  1. Supplementary Material