Research Article

A Selective Chromogenic Plate, YECA, for the Detection of Pathogenic Yersinia enterocolitica: Specificity, Sensitivity, and Capacity to Detect Pathogenic Y. enterocolitica from Pig Tonsils

Table 1

Growth and color of colonies of strains used to test the specificity of YECA media.

Strains obtained fromName of the strainsGrowth* and color of colonies on CIN plateGrowth and color of colonies on YeCM plateGrowth and color of colonies on YECA plate

Yersinia RNC from Pasteur Institute (Paris, France)Yersinia enterocolitica biotype 2 (IP383)+++++ red with a translucent rim+++++ red bull’s-eye-like+++++ small red fuchsia
Yersinia enterocolitica biotype 3 (IP29228)+++++ red with a translucent rim+++++ red bull’s-eye-like+++++ small red fuchsia
Yersinia enterocolitica biotype 4 (IP134)+++++ red with a translucent rim+++++ red bull’s-eye-like+++++ small red fuchsia
Yersinia enterocolitica biotype 1A (IP124)+++++ red with a translucent rim+++++ blue-purple+ violet colonies (5)

Collection of the Pasteur Institute (Paris, France)Yersinia aldovae (CIP103162)+++++ red with translucent rim+++++ yellow/redwith translucent rim+ small red fuchsia (1)
Yersinia bercovieri (CIP103323)+++++ red with translucent rim+++++ yellow/redwith translucent rim++ yellow/small red fuchsia
Yersinia frederiksenii (CIP80.29) +++++ red with translucent rim+++++ blue to green++ green/small red fuchsia
Yersinia kristensenii (CIP80.30)+++++ red with translucent rim++++redwith translucent rim++ pink//small red fuchsia
Yersinia massiliensis (CIP109351)+++++ red with translucent rim+++++ green++ green/small red fuchsia
Yersinia mollaretii (CIP103324)+++++ red with translucent rim+++++ yellow/redwith translucent rim+ small red fuchsia (1)
Yersinia rohdei (CIP103163)+++++ red with translucent rim+++++ yellow/redwith translucent rim+ pink (1)
Yersinia ruckeri (CIP82.80)No growthNo growthNo growth

Collection of the Pasteur Institute (Paris, France)Salmonella Typhimurium (CIP55.43)No growthNo growthNo growth
Campylobacter jejuni (CIP70.2)No growthNo growthNo growth
Enterococcus faecalis (CIP55/42)No growthNo growthNo growth
Lactobacillus plantarum (CIP103151)No growthNo growthNo growth
Pseudomonas fluorescens (CIP525)+++++ yellow+++++ yellow+ pink
Brochothrix thermosphacta (CIP103251)No growthNo growthNo growth

Field strains from Anses collectionListeria monocytogenesNo growthNo growthNo growth
Escherichia coliNo growthNo growthNo growth
Staphylococcus aureusNo growthNo growthNo growth
Klebsiella sp.No growthNo growthNo growth
Proteus mirabilisNo growthNo growthNo growth

Strains from Fondrevez et al., (2010)Morganella morganii+++++ yellow+++++ yellow++ yellow/pink
Pseudomonas sp.+++++ yellow+++++ yellow+ pink
Serratia liquefaciens+++++ pink with translucent rim+++++ green++++ green/blue/pink

*Growth was measured from no growth (absence of colonies) to 5 +++++ (important culture with numerous colonies).