Comparative Analyses of Exoproteinases Produced by Three Phytopathogenic Microorganisms

<table>Gelatinous zymograms of exoproteinases obtained by SDS-PAGE of partially purified culture media after 12 days of growth of <i>R. solani</i> (lane 1), <i>P. infestans </i>(lane 2), and <i>F. culmorum</i> (lane 3). Lane <svg height="11.175" id="M2" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 17.475 11.175" width="17.475" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.113)"><path d="M998 650l-8 -28q-71 -4 -86 -16t-22 -69l-50 -397q-3 -28 -4.5 -44t2 -29t6.5 -18.5t17 -10.5t24.5 -6.5t37.5 -3.5l-8 -28h-271l7 28q63 6 78 22t25 90l60 415h-2l-353 -552h-23l-130 536h-2l-70 -254q-44 -158 -47 -188q-5 -38 9 -51t71 -18l-6 -28h-241l8 28
q45 4 67 18.5t35 45.5q16 38 74 233l52 173q24 79 11.5 98t-89.5 26l6 28h177l136 -508l337 508h172z" id="x1D440"></path></g>
</svg> represents the molecular mass (kDa) markers as follows: phosphorylase b (94), bovine serum albumin (67), egg albumin (43), carboanhydrase (30), Kunitz soybean trypsin inhibitor (20,1), and lactalbumin (14.4). About 50 mcg of the protein were added onto the lines 1–3.</table>

Journal of Pathogens

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Figure 2

Figure 2: Comparative Analyses of Exoproteinases Produced by Three Phytopathogenic Microorganisms 