Journal of Pathogens / 2012 / Article / Tab 2

Research Article

Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis

Table 2

16S On/Off Predicted Probe Tms °C.

TargetQuasar 1 On ProbeQuasar 2 On ProbeQuasar 3 On ProbeQuasar 4 On ProbeQuasar 1 Off ProbeQuasar 2
Off Probe
Quasar 3 Off ProbeQuasar 4 Off Probe

AB50.264.918.159.550.842.418.340.3
EA69.558.611.669.761.554.518.342.1
EC69.558.611.669.761.554.53356.4
EFS24.159.7−2.870.67.834.620.6−3.2
EFM24.159.7−2.870.67.834.620.6−3.2
KP70.66613.870.662.664.53557.7
KO69.558.611.669.761.554.53356.4
PA68.966−2.85852603539.6
SA54.553.863.934.736.839.320.630.3
SE54.553.853.934.736.839.320.630.3
SH54.553.850.134.736.83820.630.3
SS54.553.858.234.736.80.920.630.3
SHM54.553.856.734.736.83820.630.3