Journal of Pathogens / 2012 / Article / Tab 2 / Research Article
Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis Table 2 16S On/Off Predicted Probe Tms °C.
Target Quasar 1 On Probe Quasar 2 On Probe Quasar 3 On Probe Quasar 4 On Probe Quasar 1 Off Probe Quasar 2 Quasar 3 Off Probe Quasar 4 Off Probe AB 50.2 64.9 18.1 59.5 50.8 42.4 18.3 40.3 EA 69.5 58.6 11.6 69.7 61.5 54.5 18.3 42.1 EC 69.5 58.6 11.6 69.7 61.5 54.5 33 56.4 EFS 24.1 59.7 −2.8 70.6 7.8 34.6 20.6 −3.2 EFM 24.1 59.7 −2.8 70.6 7.8 34.6 20.6 −3.2 KP 70.6 66 13.8 70.6 62.6 64.5 35 57.7 KO 69.5 58.6 11.6 69.7 61.5 54.5 33 56.4 PA 68.9 66 −2.8 58 52 60 35 39.6 SA 54.5 53.8 63.9 34.7 36.8 39.3 20.6 30.3 SE 54.5 53.8 53.9 34.7 36.8 39.3 20.6 30.3 SH 54.5 53.8 50.1 34.7 36.8 38 20.6 30.3 SS 54.5 53.8 58.2 34.7 36.8 0.9 20.6 30.3 SHM 54.5 53.8 56.7 34.7 36.8 38 20.6 30.3
