Differential Regulation of Escherichia coli fim Genes following Binding to Mannose Receptors

Figure 5

EIA analyses of strain NU149 grown in a pH 5.5 or 7 environment mixed with plain Sepharose beads, mannose-coated Sepharose beads, or mannose-coated Sepharose beads with 2% free mannose (wt/vol) added. Columns represent NU149 grown in pH 5.5 LB mixed with mannose-coated Sepharose beads (black column), plain Sepharose beads (white column), or mannose-coated Sepharose beads plus 2% free D-mannose (gray column) as well as NU149 grown in pH 7 LB mixed with mannose-coated Sepharose beads (left striped column), plain Sepharose beads (white dots column), or mannose-coated Sepharose beads plus 2% free D-mannose (right striped column). Optical densities at 492 (O.D.₄₉₂) were determined. The data represents the means standard deviations from at least three separate experiments. equals < 0.05 and equals < 0.0001.