Nonphotodynamic Roles of Methylene Blue: Display of Distinct Antimycobacterial and Anticandidal Mode of Actions

Figure 2

Effect of MB on cell surface phenotypes. (a) Colony morphologies of C. albicans on agar plate in absence (control) and presence of MB. (b) Relative sedimentation of C. albicans cells. Left panel shows O.D₆₀₀ for untreated (control) and MB treated cells depicted on -axis with respect to time (minutes) on -axis. Right panel shows sedimentation rates expressed as percentage and normalized by considering the untreated control as 100%. Sedimentation rates are depicted on -axis with respect to control and MB on -axis. (c) Colony morphologies of M. smegmatis on agar plate in absence (control) and presence of MB. (d) Cell sedimentation of M. smegmatis. Left panel shows O.D₅₉₀ of untreated (control) and cell treated with MB cells depicted on -axis with respect to time noted at 3 and 22 hrs on -axis. Right panel shows sedimentation rates per hour on -axis of MB treated cells with respect to control on -axis, calculated by estimating the difference in OD₅₉₀ from 0 till 22 hours per unit time interval and depicts value < 0.05.