Species A Rotavirus (RVA) Isolated from Sewage in Nigeria, 2014: Close Genetic Relatedness of Partial G, P, and NSP4 Gene Sequences Encoding G1 with Cogent Genes of Other Asian and African Rotaviruses

Figure 1

(a) Phylogenetic tree of partial VP7 (575bp) gene sequences of group A rotaviruses. Newly sequenced study strains are shown in black circles; clinical strains from Nigeria are shown by red circles. The GenBank accession numbers are indicated first in the sequence labels, bootstrap values are indicated if ≥ 70%, phylogenetic tree was constructed using the neighbor joining algorithm in MEGA 6.0 with 1,000 bootstrap replicates. Scale bar indicates number of substitutions per site. (b) Alignment of amino acid residues of rotavirus G1 sequences from sewage along Nigeria group A clinical strain NGR04 and Asian strain Hu/Cu-B1 325/KK with vaccine strain (USA/RotaTeq-W179-9/199) as reference. The blue shaded boxes represent 7-1a amino acid residues; green shaded boxes represent 7-2 amino acid residues. The brown triangle represents amino acid substitution within 7-1a and 7-2 antigenic domains.

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