Intranasal, siRNA Delivery to the Brain by TAT/MGF Tagged PEGylated Chitosan Nanoparticles
Figure 4
(a) Histopathological images of organ tissues collected 4 h following administration of the novel nanoparticle formulation containing biotin-siRNA dose at 0.5 mg/kg in animals, indicating biodistribution. The study confirmed maximum delivery of biotin-siRNA in the brain (cerebral cortex and cerebellum), with a lesser extent in the heart, kidney, liver, lungs, and stomach. The results of nanoparticle-based siRNA delivery on the left were compared to the untreated control animals on the right. (b) Quantitative analysis using Image J of the stained area in tissues from animals receiving scrambled biotin-siRNA dose, complexed in nanoparticles at 0.25 mg/kg, 0.5 mg/kg, 1 mg/kg, and 2 mg/kg, compared with the untreated control receiving 0.85% w/v NaCl. The graph shows a representative result of independent readings from two animals in each group () mean ± s.d. *** was considered highly significant based on Tukey’s post hoc analysis, when compared with other groups.
