Biological Atomic Force Microscopy for Imaging Gold-Labeled Liposomes on Human Coronary Artery Endothelial Cells
Figure 6
The internalization process of coupled liposomes was blocked when the cells were pretreated with 80 μM dynasore for 15 min before 60 min of incubation with “gold-liposomes.” The gold liposome clusters did not penetrate the cell membrane, remaining just attached to the external wall (arrow in (a)). The corresponding fluorescence image (b) shows positive signaling from the “gold liposomes” that were scanned. The cells were fixed in formalin 10% and imaged at 50 μ in contact mode in liquid (DNP-S = 12–24 kHz, N/m).
