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Journal of Pharmaceutics
Volume 2016 (2016), Article ID 5843459, 5 pages
http://dx.doi.org/10.1155/2016/5843459
Research Article

Structural Alteration in Dermal Vessels and Collagen Bundles following Exposure of Skin Wound to Zeolite–Bentonite Compound

1Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of General Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
4Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
5Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6International Branch, Shiraz University of Medical Sciences, Shiraz, Iran
7Medical Physics Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
8Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran
9Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Received 20 July 2016; Revised 21 November 2016; Accepted 27 November 2016

Academic Editor: Srinivas Mutalik

Copyright © 2016 Shahram Paydar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. This study examines the impact of one-time direct application of haemostatic agent zeolite–bentonite powder to wounded skin on the healing process in rats. Materials and Methods. 24 male Sprague-Dawley rats were randomly allocated into two groups (): (1) the rats whose wounds were washed only with sterile normal saline (NS-treated) and (2) those treated with zeolite–bentonite compound (ZEO-treated). The wound was circular, full-thickness, and 2 cm in diameter. At the end of the 12th day, six animals from each group were randomly selected and terminated. The remaining rats were terminated after 21 days. Just after scarification, skin samples were excised and sent for stereological evaluation. Results. The results showed a significant difference between the two groups regarding the length density of the blood vessels and diameter of the large and small vessels on the 12th day after the wound was inflicted. Besides, volume density of both the dermis and collagen bundles was reduced by 25% in the ZEO-treated rats in comparison to the NS-treated animals after 21 days. Conclusions. One-time topical usage of zeolite–bentonite haemostatic powder on an animal skin wound might negatively affect the healing process through vasoconstriction and inhibition of neoangiogenesis.