Adverse Effects of Subchronic Dose of Aspirin on Reproductive Profile of Male Rats

Figure 3

(a) Testis histoarchitecture of vehicle control rats (100x HE). Seminiferous tubules consisting of germ epithelium cells, various stages of spermatogonia, and spermatids as normal histoarchitecture of testis (thick arrow). Sertoli cells also exist as supporting and nutritioning cells (). Interstitial cells or Leydig’s cells existing between interseminiferous tubular spaces along with proper framing of connective tissues (star sign). (b) Testis histoarchitecture of vehicle rats (400x HE). At higher magnification, a particular seminiferous showing germ epithelium cells, sertoli cells, and various stages of spermatogonia and spermatids (thick arrow, narrow arrow, and star). (c) Testis histoarchitecture of 30-day-aspirin-subchronic-dose-treated rats (100x HE). The aspirin treatment showing cytological and nuclear degenerative changes in seminiferous tubules (thick arrow) and surrounding tissues as abnormal histoarchitecture (middle sized arrow and thin arrow). (d) Testis histoarchitecture of 30-day-aspirin-subchronic-dose-treated rats (400x HE). A 30-day treatment of aspirin caused abnormal histoarchitecture on particular seminiferous tubule as cytological toxicity by decreased numbers of various stages of spermatogonia (thin arrow). Interstitial cells showing in normal conditions as shown by middle sized arrow. Star showing degenerating stages of spermatogonia. (e) Testis histoarchitecture of 60-day-aspirin-subchronic-dose-treated rats (100x HE). Long-term aspirin treatment caused abnormal histoarchitecture as degenerations and reduced numbers of various stages of spermatogonia (middle sized arrow) and peripheral tissues (star). Thick arrow showing shrinkage in seminiferous tubules. (f) Testis histoarchitecture of 60-day-aspirin-subchronic-dose-treated rats (100x HE). 60-day aspirin treatment caused degenerative changes in particular seminiferous tubule as showing reduced numbers of various stages of spermatogonia (indicated by middle sized arrow), sertoli cells (first middle arrow), and without affecting Leydig’s cells (thin arrow).

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