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Journal of Pharmaceutics
Volume 2016, Article ID 8764274, 9 pages
http://dx.doi.org/10.1155/2016/8764274
Research Article

Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

School of Biosciences, Taylor’s University, No. 1 Jalan Taylor’s, 47500 Subang Jaya, Malaysia

Received 4 July 2016; Revised 13 September 2016; Accepted 27 September 2016

Academic Editor: Athar Ata

Copyright © 2016 Siew Ling Ong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Eleusine indica (Linnaeus) Gaertner is a traditional herb known to be depurative, febrifuge, and diuretic and has been reported with the highest inhibitory activity against porcine pancreatic lipase (PPL) among thirty two plants screened in an earlier study. This study aims to isolate and identify the active components that may possess high potential as an antiobesity agent. Of the screened solvent fractions of E. indica, hexane fraction showed the highest inhibitory activity of % at 100 μg/mL. Bioactivity-guided isolation afforded three compounds from the hexane fraction of E. indica, namely, β-sitosterol, stigmasterol, and lutein. The structures of these compounds were elucidated using spectral techniques. Lutein showed an outstanding inhibitory activity against PPL (%), with activity 60% higher than that of the reference drug Orlistat. The other compounds isolated and identified were β-sitosterol (%) and stigmasterol (%). The enzyme kinetics of E. indica crude methanolic extract on PPL showed mixed inhibition mechanism.