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Journal of Pharmaceutics
Volume 2016, Article ID 9613908, 6 pages
http://dx.doi.org/10.1155/2016/9613908
Research Article

A Comparison of the Effects of Alpha and Medical-Grade Honey Ointments on Cutaneous Wound Healing in Rats

1Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran
2Department of General Surgery, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran
3Breast Diseases Research Center, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran
4Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran
5Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
6Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Received 20 July 2016; Accepted 13 October 2016

Academic Editor: Giuseppina De Simone

Copyright © 2016 Shahram Paydar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. This study compared the healing efficacy and possible adverse effects of topical Alpha and medical-grade honey ointments on cutaneous wounds in rats. Methods. To conduct the study, 22 male Sprague-Dawley rats were randomly allocated into two equal groups: (1) rats with Alpha ointment applied to the wound surface area and (2) rats with medical-grade honey ointment applied to their wounds. The ointments were applied daily during the 21-day study period. Wound contraction was examined photographically with images taken on days 0, 7, and 21 after wounding. The healing process was histopathologically assessed using skin biopsies taken from the wound sites on days 7 and 21. Results. No statistically significant difference in mean wound surface area was observed between the two study groups. According to histopathological assessment, a significant reduction in the amount of collagen deposition ( value: 0.007) and neovascularisation ( value: 0.002) was seen in the Alpha-treated rats on day 21. No tissue necrosis occurred following the application of Alpha ointment. Conclusion. Daily topical usage of Alpha ointment on a skin wound can negatively affect the healing process by inhibiting neovascularization. Topical Alpha ointment can reduce the possibility of excessive scar formation by reducing collagen deposition.