Phototrophic Growth and Accumulation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Purple Nonsulfur Bacterium Rhodopseudomonas palustris SP5212

Table 3

Biphasic growth and PHA accumulation by R. palustris SP5212 with hydroxybutyrate and valerate as cosubstrate.

Cosubstrate	Incubation, days	Growth, g/L	PHA, % CDW	Composition of PHA, Mol%
				3HB	3HV
	6	0.42	11.26	72.57	27.42
	8	0.50	29.13	68.67	31.32
Hydroxybutyrate	10	0.61	54.17	66.59	33.41
	12	0.49	39.47	65.07	34.92
	14	0.45	37.34	57.17	42.82
	6	0.33	14.22	76.15	23.84
	8	0.38	23.20	74.05	25.94
Valerate	10	0.45	37.40	69.13	30.86
	12	0.51	29.16	67.03	32.96
	14	0.49	25.16	63.96	36.03

The isolate was grown first in malate medium for 4 days, and cells were harvested, washed, and aseptically transferred to acetate media supplemented with 0.1% hydroxybutyrate and 0.1% valerate; other conditions are the same as under Table 1.