In Vitro Activity of Geldanamycin Derivatives against Schistosoma japonicum and Brugia malayi
Figure 2
(a). Survival of paired adult male and female Schistosoma japonicum in the presence of human red blood cells and five micromolar geldanamycin (GA, triangles) or five micromolar 17-AAG (squares) compared to control parasites supplemented only with human red blood cells (diamonds). 50%–60% mortality was achieved at 24 hours while 90% of controls remained viable (). (b). Survival of individual male worms. 50%–60% mortality was achieved at 24 hours while 90% of controls remained viable (). (c). Survival of individual female worms. 35%–50% mortality at 24 hours while 95% of controls remained viable (). At 72 hours 17-AAG and GA caused 50% greater mortality versus controls. D. Survival of individual male and female worms cultured together. GA, male, star. GA female, circle. 17-AAG male, triangle. 17-AAG female, cross. At 36 hours GA caused 60% mortality in male worms while 90% of controls remained viable (). At 72 hours 90%–100% of GA and 17-AAG treated worms were dead versus 50% mortality in controls (). For (a)–(d), the data represent the mean ± S.D. of triplicate determinations in a representative experiment.
