Research Article

Inhibition of the Growth of Plasmodium falciparum in Culture by Stearylamine-Phosphatidylcholine Liposomes

Table 1

Effects of Various Liposomes on the Viability of Plasmodium falciparum in vitro.

Types of liposomesIC50 in terms of phospholipid concentration (μM)

SPC>208.33
SPC: PA>208.33
SPC: SA24.08 ± 1.15
SPC: Stearic acid (octadecanoic acid)>208.33
SPC: Stearyl alcohol (octadecyl alcohol)>208.33
SPC: myristic acid ( tetradecanoic acid)>208.33
SPC: Palmitic acid (hexadecanoic acid)>208.33
SPC: myristyl alcohol (tetradecyl alcohol)>208.33
SPC: Cetyl alcohol (hexadecyl alcohol)>208.33
Free spermidine>1102

Cells with 1% parasitemia and 2% hematocrit in the presence of various concentrations of above mentioned liposomal formulations (6.5 μM–208 μM lipids) and free spermidine (34 μM–1102 μM) were treated for 42 h at 37°C (in between at 24 h, [3H] hypoxanthine was added). The cell-associated radioactivity was determined and inhibition of growth calculated by comparison with controls (without liposomes/spermidine).