An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents

Table 1

Reactions of sera from dogs suffering from tick paralysis to Ixodes holocyclus (I.h) and Rhipicephalus (Boophilus) microplus (R.m) antigen in ELISA.

	Day 0^1		Day 16^2
Dog ID	I.h	R.m	I.h	R.m
A	0.666^3	0.518	n/a	n/a
B	0.536	0.477	0.515	0.443
C	1.227	0.706	2.223	1.358
D	1.902	1.13	1.769	0.993
E	0.41	0.348	0.43	0.359
F	0.538	0.335	0.536	0.363
G	0.565	0.318	0.828	0.362
H	1.047	1.05	2.068	1.201

¹Sera were collected on day of admission prior to the administration of TAS.
²Sera were collected approximately 16 days after TAS was administered.
³ Optical density (405 nm) of ELISA reaction of sera (1/50) to Ixodes (I.h) and Rhipicephalus (R.m) antigen.