An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents
Table 2
The reaction of sera from rats exposed to Ixodes holocyclus ticks with Ixodes holocyclus and Rhipicephalus (Boophilus) microplus antigens in ELISA.
Rat ID
ELISA
Ixodes antigen
Rhipicephalus antigen
Preinfestation*
Postinfestation*
Preinfestation
Postinfestation
A
0.19 ± 0.04
0.29 ± 0.005
0.08 ± 0.002
0.07 ± 0.005
B
0.15 ± 0.02
0.31 ± 0.004
0.11 ± 0.003
0.15 ± 0.007
C
0.2 ± 0.01
0.25 ± 0.004
0.13 ± 0.06
0.05 ± 0.004
D
0.2 ± 0.05
0.4 ± 0.002
0.08 ± 0.002
0.16 ± 0.001
E
0.11 ± 0.03
0.2 ± 0.007
0.08 ± 0.006
0.08 ± 0.007
F
0.14 ± 0.02
0.25 ± 0.005
0.068 ± 0005
0.14 ± 0.003
G
0.17 ± 0.06
0.3 ± 0.005
0.1 ± 0.004
0.12 ± 0.003
Tick Naïve Rat
0.11 ± 0.03
0.09 ± 0.001
0.11 ± 0.004
0.07 ± 0.004
*Sera were collected from individual rats before and 21 days after tick infestation, diluted 1/50, and tested against Ixodes and Rhipicephalus antigens in ELISA using the optimised conditions and protocol described earlier.
