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Journal of Parasitology Research
Volume 2012 (2012), Article ID 278028, 6 pages
http://dx.doi.org/10.1155/2012/278028
Clinical Study

Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo

1Département de Médecine Tropicale, Maladies Infectieuses et Parasitaires, Cliniques Universitaires de Kinshasa, Université de Kinshasa, 747 Kinshasa XI, Democratic Republic of Congo
2Service de Parasitologie-Mycologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris et Faculté de Médecine Lariboisière-Saint-Louis, Université Paris VII, 75010 Paris, France
3Faculty of Health Sciences, Walter Sisulu University, Private Bag XI, Mthatha, Eastern Cape 5117, South Africa
4Department of Internal Medicine, University of Kinshasa, 783 Kinshasa XI, Democratic Republic of Congo
5National Center for Malaria Research, AP-HP, CHU Pitié Salpetrière, 75013 Paris, France
6AP-HP, Groupe hospitalier Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Université Pierre et Marie Curie, 75013 Paris, France

Received 19 March 2012; Revised 10 April 2012; Accepted 24 April 2012

Academic Editor: Francisca Mutapi

Copyright © 2012 Roger Wumba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients. Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including E. bieneusi and E. intestinalis was performed in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E. bieneusi. PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard. Results. Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E. bieneusi was 7.9% ( 𝑛 = 1 9 ) among the 19 E. bieneusi, one was coinfected with E. intestinalis. In 19 E. bieneusi persons using PCR-RFLP method, 5 type I strains of E. bieneusi (26.3%) and 5 type IV strains of E. bieneusi (26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%. Conclusion. Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.