Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients
Subsets of monocytes and co-stimulatory molecules expression in monocytes of CL patients () stimulated in vitro with SLA in the presence or absence of S. mansoni antigens rSm29 and rSmTSP-2 or with a fraction of S. mansoni soluble adult worm antigen (SWAP) PIII. Frequency of classical (CD14++CD16−), intermediate (CD14++CD16+) and nonclassical (CD14+CD16++), monocytes (A), and expression of HLA-DR, CD80, and CD86 in classical, intermediate and nonclassical monocytes of cutaneous leishmaniasis patients (B–D). Cells were stained for surface expression of CD14, CD16, HLA-DR, CD80, and CD86 using flow cytometry. cultures without stimulation (WS) versus SLA + S. mansoni antigens; SLA versus SLA + S. mansoni antigens (Wilcoxon matched pairs test).