Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene
Figure 1
Effect of immunization with pcDNA-3-1E-mChIL-15 on spleen lymphocyte proliferation. Seven-day-old chickens were divided into three groups. Chickens in each group were, respectively, intramuscularly immunized with 100 μg pcDNA-3-1E-linker-mChIL-15 (group 1), 100 μg pcDNA3.1 (group 2), and sterile TE buffer (pH 7.6) (group 3) at 14 days of age. A booster immunization was given at 21 days of age. All chickens were inoculated orally with 5 × 104 sporulated oocysts of E. acervulina at 28 days of age (day 7 after secondary immunization). The proliferative response of spleen lymphocytes to stimulation with Con A was measured by MTT methods at day 28 and 34 days of age (day 6 after challenge). Data were expressed as means ± standard errors ( per group). Bars not sharing the same small letter are significantly different according to Duncan’s multiple range test () in each time point.
