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Journal of Parasitology Research
Volume 2012, Article ID 929743, 11 pages
Review Article

ETosis: A Microbicidal Mechanism beyond Cell Death

1Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), 21941-901 Rio de Janeiro, RJ, Brazil
2Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), 21941-901 Rio de Janeiro, RJ, Brazil
3Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), 23890-000 Seropedica, RJ, Brazil

Received 30 August 2011; Accepted 10 November 2011

Academic Editor: Dario Zamboni

Copyright © 2012 Anderson B. Guimarães-Costa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Video microscopy of the neutrophil-Leishmania amazonensis interaction. Human neutrophils (N) were incubated with stationary-phase (P) promastigotes (1N:5P ratio) and NETs were stained with Sytox green. The interaction was recorded during 2:55 h using an epifluorescence microscope (Zeiss AxioObserver) equipped with Colibri ilumination LED system. Frames of live Leishmania-neutrophil interaction were acquired in high resolution mode using definitive focus correction, differential interference contrast (DIC) and green fluorescent excitation filter. White arrow indicates a NET-trapped promastigote. Note that netosis visualized as a green staining is increasing over time. Bar, 5 µm.

  1. Supplementary Material