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Journal of Parasitology Research
Volume 2012, Article ID 947948, 8 pages
Research Article

Cross-Reaction between the Crude Hydatid Cyst Fluid Antigens of Human and Animals Origin in Response to Human IgG Class and Subclasses

1Immunology Department, Faculty of Medicine, Ilam University of Medical Sciences, Ilam 6931994585, Iran
2Clinical Microbiology Research Center, Ilam University of Medical Sciences Iran, Ilam 6931994585, Iran
3Parasitology Department, Faculty of Veterinary Sciences, Ilam University, Ilam 6931994585, Iran

Received 7 November 2011; Revised 31 December 2011; Accepted 2 January 2012

Academic Editor: Michael Lanzer

Copyright © 2012 Afra Khosravi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The current work aimed to evaluate the cross-reactivity of human immune sera against crude hydatid fluid antigens of sheep, human, mouse, cattle, as well as B fraction of cystic fluid antigen. 30 balb/c mice were infected with sheep hydatid cyct fluid antigen containing protoscolex after the viability of these protoscolices was assessed. ANOVA was used to test the difference of themean of optical density (OD) values among case and control groups. The highest human IgG class antibody was against antigen B (0.93) and the lowest against cattle HCF antigen (0.32). The differences between responses to these antigens were statistically significant (P<0.001). The sensitivity and specificity of ELISA test used for evaluating the responses of human total IgG to different hydatid cyst fluid (HCF) antigens among the case and control groups were 100 and 95.8%, respectively. Cross-reaction of human IgG class and subclasses responses was found almost for all the antigens with the best reaction against human and cattle (HCF) antigens and antigen B using a ratio of mean OD value to each antigen divided by the cut-off point value for the same antigen. Human sera showed a considerable cross-reactivity against all antigens by using ELISA.