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Journal of Parasitology Research
Volume 2013, Article ID 591520, 9 pages
Research Article

Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells

Institute of Zoology, Technische Universität Dresden, Helmholtzstraße 10, 01062 Dresden, Germany

Received 18 April 2013; Accepted 3 June 2013

Academic Editor: Renato A. Mortara

Copyright © 2013 Hong Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Variations of fluorescence pattern within a gamont cluster. A: The YFP fluorescence shows four parasite stages side by side. Only the exterior stages are showing a strong nuclear signal. The both internal stages do not show such a nuclear signal. B: The TDT fluorescence pattern indicates the exterior stages as macrogamonts by the gam56 promotor driven specific TDT expression. The internal stages do not show TDT expression. C: Overlay of A and B. D: Overlay of C and a transmitted light micrograph shown in E. Through the absence of TDT fluorescence and as well as nuclear signals, the internal stages can be interpreted either as young macrogamonts still without gam56 expression, or untypical schizonts without accentuated nuclear YFP signals, or even microgamonts. Shi et al. (2008) described the nuclei of microgametes in immature microgamonts as not clear. Finally the status of the shown internal stages between the exterior macrogamonts remains unclear. Bar: 20 μm.

Supplementary Figure 2: DAPI fluorescence signal corresponding to Figure 3 H shows nuclei of host cells and parasitic stages at 144 h p.i. Bar 50 μm.

Supplementary Figure 3: Image sequence of 31 (11 shown) micrographs illustrate the movement of a third generation merozoite in a small bowel tissue smear (148 h p.i.) The long, straight merozoites moved forward about two times of its own length and then backward about 3 times of its own length. Bar: 25 μm.

  1. Supplementary Figures