Improved Method for the Detection and Quantification of Naegleria fowleri in Water and Sediment Using Immunomagnetic Separation and Real-Time PCR

Table 3

N. fowleri detection in seeded 1 L lake water samples.

Seed level	Direct PCR without culture				Culture followed by PCR confirmation
	IMS		No IMS		IMS		No IMS
	CT	Pos^† no.	CT	# Pos	CT	Pos no.	CT	# Pos
14	39.1	1	38.0	1	Negative	0	38.9	1
63	38.5	3	40.5	2	32.8	1	34.6	2
388	35.1	4	36.7	4	27.8	4	38.3	1
4,900	31.9	4	33.2	4	28.9	4	35.7	4
11,300	29.83	4	29.96	4	23.99	4	34.87	4
129,000	27.87	4	28.19	4	25.38	4	31.57	4

†Number of positive results out of four replicate tests (all conditions were repeated four times).