Mast Cell Subsets and Their Functional Modulation by the Acanthocheilonema viteae Product ES-62
Figure 4
Calcium mobilisation in mast cell subsets. Fura-2/AM-loaded resting, nonsensitised (a) & (b) or anti-DNP IgE (0.5 μg/mL)-sensitised (a)–(h) mucosal-type BMMC (e) & (f), CTMC (g) & (h) and PDMC (a)–(d) were stimulated at 50 s with DNP (0.5 μg/mL) to induce cross-linking (XL) of FcεR1 (a), (c), (e) & (g) or 0.5 μg/mL LPS (b), (d), (f) & (h), and intracellular calcium mobilisation and influx recorded in real time using excitation-emission ratios of 340/380 nm (a)–(h). For analysis of intracellular mobilisation alone, the cells were stimulated in calcium free HBSS supplemented with 100 μM EGTA to remove all extracellular calcium (EGTA). Calcium levels were calculated from and values and the data are presented as the mean calcium values of triplicate samples (base line calcium values subtracted) from a single experiment representative of at least 3 independent experiments.
